LncRNA–miRNA–mRNA ceRNA network of different body colors in Plectropomus leopardus

Long non-coding RNAs (lncRNAs) play a multifaceted role in transcriptional regulation, and the potential molecular regulatory mechanisms of lncRNAs lncRNA–miRNA–mRNA networks body color formation are great significance for its selective breeding. Therefore, lncRNA-miRNA-mRNA ceRNA network red- black-colored Plectropomus leopardus were identified analyzed. Sequencing analyses 167 differentially expressed (DELs) between P. , including 89 upregulated 78 downregulated DELs red-colored group (false discovery rate (FDR) < 0.05 |log 2 FC| > 1). Differentially miRNA (DEM), genes (DEG), DEL found 605 125 negatively co-expressed miRNA–mRNA pairs lncRNA–miRNA pairs, respectively. Further correlation analysis with Spearman’s coefficient >0.9 as threshold 3,721 lncRNA–mRNA pairs. Then, competitive endogenous RNA (ceRNA) 325 (p 0.05) was obtained. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment DEGs showed that melanin metabolic process, lipid metabolism, immune-related enriched. The provided interactions among lncRNAs, miRNAs, mRNAs extended foundation formation.